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KMID : 0379120060340020092
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Korean Journal of Mycology
2006 Volume.34 No. 2 p.92 ~ p.97
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Antimicrobial Activity of the Coriolus versicolor Liquid Culture Extracts Against Antibiotic Resistant Bacteria and Purification of Active Substance
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Lee Jung-Sun
Kim Taek
Lee Yoon-Hee
Kim Sung-Min
Kim Hyun-Gul
Kim Woo-Jung
Oh Duk-Chul
Park Yong-Il
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Abstract
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The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.
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KEYWORD
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Antibacterial activity, Antibiotic resistant bacteria, Coriolus versicolor Ethylacetate extract
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